eCM Meeting Abstracts 2015, Collection 3
Abstracts of the Tissue and Cell Engineering Society (TCES)
meeting
on 19-21 July 2015 at Southampton.
ORAL PRESENTATIONS
All abstracts: click here to download all the abstracts in one file
Page 1: C Murray-Dunning, L Thanabalasundaram, AGE Day, N Vysokov, J Sinden, L Stevenato, JB Phillips
Differentiated CTX human neural stem cells adopt a glial phenotype in RAFT-stabilised collagen hydrogels suitable for nerve tissue engineering
Page 2: JC Bridge, GE Morris, JW Aylott, MP Lewis, FRAJ Rose
Developing an in vitro model of smooth muscle contraction
Page 3: V Parikh, M Miraftab, A Hidalgo, M Azzawi
Design and development of novel electrospun small diameter vascular prosthesis
Page 4: JM Kanczler, D Gothard, EL Smith, LJ White, O Qutachi, KM Shakesheff, ROC Oreffo
The capacity of growth factor releasing hydrogels to regenerate bone in an ex vivo chick femur defect model
Page 5: LE Clarke, JC McConnell, MJ Sherratt, SM Richardson, JA Hoyland
Effects of GDF6, hypoxia and load on mesenchymal stem cell discogenic differentiation and tissue engineered construct composition and micromechanics
Page 6: CC Moura, JP Smus, RS Tare, ROC Oreffo, S Mahajan
Assessing early skeletal stem cell differentiation into adipocytes using coherent anti-Stokes Raman scattering microscopy
Page 7: JE Dixon, G Osman, G Morris, C Denning, KM Shakesheff
GET: Glycosaminoglycan (GAG)-binding Enhanced Transduction of Functional Proteins
Page 8: G Gigliobianco, L Dew, S MacNeil
Developing a layer-by-layer biomaterials coating approach with heparin and patient’s blood to induce angiogenesis
Page 9: US Jonnalagadda, P Glynne-Jones, ROC Oreffo, M Hill, RS Tare
Design and validation of multiwell acoustofluidic bioreactor platform for tissue engineering
Page 10: M Bennett, F Bathawab, M Cantini, J Reboud, M Salmerón-Sánchez
Polymer surface mobility – a means to drive cellular behaviour through manipulating extracellular matrix organisation
Page 11: RM Delaine-Smith, GC Reilly
Does scaffold fibre alignment guide differentiation and matrix production by osteogenic progenitors?
Page 12: LA Ruiz Cantu, A Gleadall, C Faris, J Segal, J Yang, K Shakesheff
Bioprinting of personalised tissue engineered cell constructs for nasal reconstruction
Page 13: J Clarke, A Harding, F Boissonade, JW Haycock. F Claeyssens
3D printing and plasma functionalisation of peripheral nerve guides
Page 14: E Budd, T Sanchez-Elsner, ROC Oreffo
Role of miR-146b-5p in chondrogenesis
Page 15: N Peake, S Thorpe, B Pinguaan-Murphy, DA Lee, AJ Hobbs, TT Chowdhury
Differential signalling through natriuretic peptide receptors mediates the chon-drogenic effects of C-type natriuretic peptide
Page 16: S Inglis, D Christensen, DI Wilson, JM Kanczler, ROC Oreffo
Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis
Page 17: H Markides, J McLaren, JR Henstock, N Telling, B Scammell, K Shakesheff, AJ El Haj
Remote cell activation for bone regeneration – a preclinical animal study
Page 18: RC Ching, M Wiberg, PJ Kingham
Stem cell-derived exosomes: a new tool to treat nerve injuries?
Page 19: TP Dale, A DeCastro, EK Parkinson, NJ Kuiper, NR Forsyth
Cell line creation using hTERT immortalisation impacts on sulphated glycosaminoglycan secretion and chondrogenic potential
Page 20: I Moreno, G Hulsart-Billströ1, S Lanham, ND Evans, ROC Oreffo
Examination of human bone tissue regeneration using the chorioallantoic membrane (CAM): an ex vivo replacement model for animal research
Page 21: G Bouet, M Colzani, M Arumugam, D Howard, T Moreau, W Afshar-Saber, S Best, R Cameron, C Ghevaert
In vitro recreation of the 3-dimensional bone marrow niche for platelets production
Page 22: JR Henstock, JCFA Price, AJ El Haj
Dynamic hydrostatic pressure – an osteogenic stimulus for mesenchymal stem cells in 3D hydrogel culture
POSTERS
Page 23: A Aied, P Mistry, K Shakesheff, J Yang
3D Bioprinting of soft-tissue substitutes from cell-seeded stimuli responsive hydrogels
Page 24: AM Aladdad, LJ White, C Alexander, FRAJ Rose
Creating thermo-responsive co-electrospun fibres for 3D cell growth
Page 25: B Al-Azzawi, C Kelly, NR Forsyth
Stem cell product-based blockage of cytokine-induced apoptosis: implications for diabetes
Page 26: N Alom, KM Shakesheff, LJ White
Hydrogel scaffold derived from decellularized bone matrix with osteogenesis-inducing activity and protein carrier ability for bone regeneration
Page 27: MA Al-Zubaidi, MR Siddique, J Sulé-Suso, NR Forsyth
Determination of headspace volatile levels: potential roles in mesenchymal stem cell biology
Page 28: MH Amer, LJ White, FRAJ Rose, KM Shakesheff
Quantifying the impact of delivery of mesenchymal stem cells using narrow-bore needles for cell therapy applications
Page 29: HJ Anderson, JN Roberts, J Sahoo, CC Berry, ROC Oreffo, R Uljin, MJ Dalby
Enzyme responsive technology for mesenchymal stem cell control
Page 30: R Angus, CO’Rouke, A Michael-Titus, JB Phillips
Focal mechanical injury induces astrogliosis in a 3D tissue engineered culture model
Page 31: J Appelt, AD Metcalfe, G Phillips,YH Martin
Advanced scaffolds for adipose tissue reconstruction
Page 32: A Baki, C Rahman, O Qutachi, KM Shakesheff
Surface tunable injectable microspheres for cell delivery applications
Page 33: L Balakrishnan, FV Lali, AD Metcalfe, YH Martin
Effect of hypoxia on the expression of miR-21, miR-205 and miR-210 in primary keratinocytes
Page 34: K Bardsley, Y Yang, AJ El Haj
Fluorescently monitoring poly(lactic-co-glycolic acid) degradation and its effect on cellular osteogenic potential
Page 35: P Battersby, A Sloan, R Waddington
Examining the effect of culture expansion on mesenchymal stem cells isolated from rat femoral compact bone
Page 36: D Bellamy, H Alsaykhan, JZ Paxton
Development of a novel culture system to investigate cellular interactions at engineered tissue interfaces
Page 37: R Balint, S Richardson, SH Cartmell
Low density sub-culturing is essential for maintaining the self-renewal and differentiation potential of MSCs
Page 38: LA Bosworth, P Bhaskar, MA O’Brien, H Kriel, E Smit, SH Cartmell
Comparison of manufacture and sterilisation techniques on in vivo biological response: a six-week study using electrospun yarns grafted into mouse tendons
Page 39: A Chhatwal, S Przyborski
The impact of prolonged 3D growth on cytoskeletal re-organisation, adhesion signalling and cell metabolism
Page 40: SS Chowdhury, O Qutachi, FRAJ Rose, KM Shakesheff
Membrane emulsification : a platform to fabricate injectable porous PDLLGA microparticles
Page 41: BDM Coverdale, JE Gough, JA Hoyland
Modification of electrospun nanofibrous scaffolds for improved cellular adhesion and biocompatibility
Page 42: RH Coy, JB Phillips, RJ Shipley
A mathematical model with the capacity to direct and accelerate the design of cellular peripheral nerve repair conduits
Page 43: EM Czekanska, J Geng, ND Evans, M Bradley, ROC Oreffo
Directing cell differentiation through delivery of PLGA-nanoparticles loaded with bioactive molecules
Page 44: AGE Day, KS Bhangra, C Murray-Dunning, L Thanabalasundaram, N Grace, G Cameron, L Stevanato, J Sinden, JB Phillips
Hypothermic and cryogenic preservation of artificial neural tissue made using differentiated CTX human neural stem cells in collagen gels
Page 45: MC de Andrés, ROC Oreffo
Understanding the epigenetic mechanisms underlying anabolic and catabolic gene regulation in osteoarthritis
Page 46: S Desissaire, OG Andriotis, PJ Thurner
Nanoelasticity and structure of collagen fibrils highly influenced by osmotic stress
Page 47: M Dunn, M Kamudzandu, R Fricker, P Roach
Designing and fabricating a functional in vitro neural circuit
Page 48: HM Eltaher, J Yang, JE Dixon, KM Shakesheff
Assessment of the gradient delivery of bioactive molecules to hydrogel-encapsulated cells using flow cytometric analysis
Page 49: A Elttayef, L Lyu, C Kelly, Y Yang
The effect of substrate coating on pseudoislet formation
Page 50: T Eriksson, S Fedele, R Day, V Salih
Examining tissue invasion in an organotypic ameloblastoma model
Page 51: PP Esteban, C Moore, A Scott, MJ Ellis
Engineering a capillary bed bioreactor for dermal bioavailability and toxicity testing: caffeine permeation across novel hollow fibre membranes
Page 52: AR Fernandes, DM Chari
DNA minicircle vector technology: a new solution to overcome plasmid size restrictions on magnetic particle based gene delivery to neural stem cells
Page 53: R Gam, J Norden, R Crossland, K Pearce, C Lendrem, E Holler, R Dressel, AM Dickinson
MICA genotype, serum and expression level effects on the outcome of HSCT
Page 54: J Garcia, C Mennan, J Richardson, S Roberts, K Wright
Comparison of the potential of human chondrocytes, bone marrow, adipose and synovial fluid derived mesenchymal stem cells from arthritic joints for osteochondral repair
Page 55: M Georgiou, JD Reis, R Wood, D Li, D Choi, I Wall
Towards an allogeneic olfactory ensheathing cell line for spinal cord injury
Page 56: A Giacaman, D Pritchard, A Cameron, FRJ Rose
Biological active compound from Green propolis for wound healing
Page 57: DM Gibbs, CRM Black, G Hulsart-Bilstrom, ROC Oreffo, JI Dawson
Bone formation at physiological doses of BMP through clay hydrogel localisation
Page 58: A Glen
Cloning rings do not necessarily generate cellular clones, implications and recommendations for stem cell tissue engineering
Page 59: A Glen, L Zilic, C Taylor, R Diez-Ahedo, X Bazan, F Claeyssens, JW Haycock
MicroCT facilitated analysis and design of next generation nerve guide conduits
Page 60: P Goggin, KC Zygalakis, ROC Oreffo, P Schneider
3D imaging of osteocytes using serial block-face scanning electron microscopy
Page 61: E Grigoriou, M Cantini, MJ Dalby, M Salmeron-Sanchez
Graded organisation of fibronectin on polymer surfaces to tune cell response
Page 62: RB Gurden, P Sweeten, LA Turner, CC Berry, M Salmeron-Sanchez, ROC Oreffo, MJ Dalby
Towards an in vitro stem cell niche using surface chemistry and mesenchymal stem cells
Page 63: OA Hamid, J Yang, HM Eltaher, KM Shakesheff
Three dimensional printing of hydrogels, growth factors and mammalian cells to create a biomimetic environment for nervous tissue formation in vitro
Page 64: X He, SE Thomson, AL Bernassau, SJ Moug, MO Riehle
Isolation of colonic smooth muscle cells from neonatal rats: Can ultrasound induce contractions?
Page 65: JR Henstock, M Rotherham, AJ El Haj
Remotely controlled mechanotransduction via magnetic nanoparticles: applications for injectable cell therapies
Page 66: SG Hulsart Billström, S Inglis, ROC Oreffo
Screening growth factor loading capacity of distinct biomaterials using a high-throughput ex vivo avian model
Page 67: S Inglis, JM Kanczler, SG Hulsart Billström, ROC Oreffo
3D Human bone marrow and endothelial cell pellets induce osseointegration and differentiation in organ cultures of E18 chick femur drill defects
Page 68: AA Janeczek, MH Horrocks, RS Tare, E Scarpa, TA Newman, ROC Oreffo, SF Lee, ND Evans
Association of Wnt3A growth factor with PEGylated liposomes for bone tissue regeneration
Page 69: JM Jones, DJ Player, NRW Martin, R Brown, MP Lewis , V Mudera
Muscle organoid standardization: an in vitro 3-D study
Page 70: AG Kay, S Parida, A Stefan, L Rump, J Middleton, O Kehoe
Assessment of aggrecanase- and MMP-driven cartilage degradation following MSC treatment for Rheumatoid Arthritis
Page 71: D Kredi, MO Riehle
Physical substrate factors, NGF concentration and Rho-ROCK inhibition affect outgrowth response in individual neurons
Page 72: JK Kular, MJ Ellis, RI Sharma
Adult and foetal tenocytes display phenotypic variation in relation to cell attachment, spreading and their cytoskeleton
Page 73: D Kumar, VL Workman, LC Diaz, F Rose, A Miller, A Saiani, JE Gough
Injectable peptide hydrogel for the treatment of Barrett’s oesophagus
Page 74: AK Kureshi, M Dziasko, JL Funderburgh, JT Daniels
3D culture model of human corneal epithelial-stromal cell interactions in the limbal niche
Page 75: C Lee-Reeves, JB Phillips, C O’Rourke
Development of a tissue engineered co-culture model to study neurodegeneration
Page 76: N Lewis, E Lewis, M Dalby, C Berry
The magnetic labelling of stem cells to develop a functional hematopoietic stem cell niche in vitro
Page 77: T Li, T Jenkins, SA Lanham, D Sreenivasan, J Fernandez, PJ Thurner, ROC Oreffo
In utero vitamin D depletion reduces femural bone area and strength of offspring at 21 days of age
Page 78: KA Luetchford, JB Chaudhuri, PA De Bank
Silk fibroin/gelatin blended materials as osteogenic scaffolds for tissue engineering
Page 79: N Madarbux, S Shafaie, M Bloomfield, MB Brown, V Hutter, DYS Chau
A simplified, in vitro, 3D printed dry eye model and its application in the assessment of therapeutic ocular lubricants
Page 80: YH Martin, AD Metcalfe
A serum-free and feeder-free protocol for expanding human keratinocytes on biodegradable microcarriers for the treatment of severe burn injuries
Page 81: NRW Martin, DJ Player, MC Turner, MP Lewis
Tissue engineered skeletal muscle exhibits hypertrophy in response to supplementation with the amino acid L-Leucine
Page 82: JS McLaren, H Markides, JR Henstock, BE Scammell, KM Shakesheff, AJ El Haj
The use of magnetic nanoparticles to encourage new bone growth in a large animal model
Page 83: MM Merkhan, NR Forsyth
The role of hMSCs-secretome in attenuating the immune response using Jurkat cells as an in vitro model
Page 84: G Osman, C Denning, KM Shakesheff, JE Dixon
Novel GET proteins for efficient gene transfer
Page 85: DDG Owens, SA Przyborski
Modelling the development of human tissue in vitro from pluripotent stem cells
Page 86: HA Owida, N Kuiper, Y Yang
Exploration of new anisotropic scaffolds for articular cartilage regeneration
Page 87: DJ Page, JI Dawson, R Mani, ROC Oreffo, CE Clarkin, ND Evans
Novel clay gels as regenerative microenvironments for the treatment of diabetic foot ulcers
Page 88: MM Pardo-Figuerez, NRW Martin, DJ Player, SDR Christie, MP Lewis
On course to the next generation of prosthetics: Chemical functionalised substrates for neuronal guidance
Page 89: C Peticone, JC Knowles, M Micheletti, JJ Cooper-White, IB Wall
Ti/Co doped phosphate bioactive glass microcarriers: effect on hBM-MSCs osteogenic and angiogenic responses
Page 90: DJ Player, NRW Martin, MP Lewis
Mechanical unloading induces tissue and cellular responses in engineered skeletal muscle
Page 91: J Price, JR Henstock, AJ El Haj
Hydrostatic preconditioning of hMSC-collagen scaffolds enhances fracture repair in an ex vivo nonunion defect
Page 92: RH Quelch, SA Przyborski
Impact of substrate topography on the growth of pluripotent stem cells cultured in three dimensions
Page 93: Y Reinwald, AJ El Haj
Mechanical and topographical cues affecting the stem cell fate for bone tissue engineering
Page 94: R Ribeiro, AM Ferreira, K Dalgarno
Osteosarcoma cell encapsulation for biomedical applications
Page 95: M Rotherham, JR Henstock, O Qutachi, KM Shakesheff, AJ El Haj
Regulation of Wnt signalling and promotion of bone mineralisation using peptide-conjugated magnetic nanoparticles
Page 96: K Sanen, R Paesen, W Martens, S Luyck, JB Phillips, I Lambrichts, M Ameloot
Label-free monitoring of cell-seeded collagen type I hydrogels reveals marked changes in extracellular architecture over time
Page 97: E Scarpa, AA Janeczek, ROC Oreffo, TA Newman, ND Evans
Polymeric-nanoparticles for the delivery of small molecules during bone fracture repair
Page 98: RJ Shipley, RH Coy, T Evans, OR Evans, G Kennedy, D Hodgson, JB Phillips
Mathematical models as a tool to direct the spatial distribution of cells and materials in tissue-engineering conduits for peripheral nerve repair
Page 99: LE Sidney, MJ Branch, OD McIntosh, K Selwyn, CY Lee, A Hopkinson
Amniotic membrane as a tissue engineering substrate for corneal-derived mesenchymal stem cells
Page 100: LE Sidney, HS Dua, A Hopkinson
Investigation into the importance of maintaining CD34 expression in corneal stroma-derived stem cells
Page 101: KE Sisti, MC de Andrés, DA Johnston, AC Guastaldi, ROC Oreffo
Evaluation of human skeletal progenitor cell function modulated by laser titanium surface modification
Page 102: A Takahashi, MC de Andrés, K Hashimoto, E Itoi, ROC Oreffo
Epigenetic regulation of interleukin-8, an inflammatory chemokine, in osteoarthritis – a potential therapeutic target
Page 103: D Thomas, C Matthews, RA Brown
3D Collagen model using static compressive loading for assessing cell-mediated mineralization
Page 104: SD Thorpe, A Gowran, JH Kim, DA Lee
Endocannabinoids can prevent interleukin-1β-induced cartilage degradation while enhancing mesenchymal stem cell chondrogenesis
Page 105: I Tundidor-Peréz, P Patel, DYS Chau
A technique to enhance the desiccation tolerance of mammalian cells: exploitation of novel proteins from Deinococcus radiodurans
Page 106: MC Turner, DJ Player, NRW Martin, MP Lewis
The effects of insulin upon skeletal muscle tissue engineered collagen constructs
Page 107: SL Wilson, AP Lynch, M Ahearne
Dextran use in corneal decellularisation: how, where, and if?
Page 108: NM Wragg, DJ Player, NRW Martin, Y Liu, MP Lewis
Preclinical musculoskeletal junction testbed: co-culture of 3D tissue engineered skeletal muscle and bone constructs
Page 109: JM Xavier, P Rosendahl, D Spencer, O Otto, J Guck, ROC Oreffo, H Morgan
Skeletal stem cell sorting: characterisation of physical properties for microfluidic separation applications
Page 110: AYF You, S Bertazzo, JP St-Pierre, JAM Steele, AH Chester, MH Yacoub, MM Stevens
Characterisation of human cardiovascular tissue in calcific disease using Raman spectroscopy
Page 111: T Zhou, ED McCarthy, C Soutis, SH Cartmell
Design of scaffold morphology for optical cell differentiation using novel polymerisation techniques
Page 112: L Zilic, JW Haycock, S-P Wilshaw
Development of an in vitro peripheral nerve model using xenogeneic nerve tissue
All abstracts: click here to download all the abstracts in one file